Fig. 8

Decreased seizure-induced glial activation in the retina after CX3CR1 antibody treatment. Representative photomicrographs of the retina 7 weeks after SE (a) and CX3CR1 antibody-treated SE (b). Arrow heads depict Iba1⁺ cells in the IPL and OPL. Quantification of numbers of Iba1⁺cells in the ipsi- and contralateral retina at 7 weeks revealed a decrease after CX3CR1 treatment (c). Quantification of the relative percentage of microglia with different morphologies at 7 weeks showed a relative increase in ramified and a reduction in intermediate and amoeboid morphologies (d). Representative images of GFAP expression in macroglia in the retina 7 weeks post-SE (e) and post-SE with CX3CR1-treatment (f). GFAP⁺ processes in the IPL are marked with arrow heads in (e). Quantification of GFAP⁺ Müller cell processes in the IPL showed reduced numbers after SE (g). Data are presented as means ± SEM in (c) and (d), and as median range with upper quartile range in (g), n = 6 SE and n = 7 CX3CR1-treated SE group. *p ≤ 0.05 un-paired t test in (c), 2-way ANOVA in (d), Mann-Whitney’s rank sum test in (g). †p = 0.067 in (c). Scale bars are 500 μm for (a) and (b), and 50 μm for (e) and (f)